BioTek: Microplate Instrumentation & Software Solutions
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Lavatore di micropiastre 405 Touch

The new 405™ Touch Microplate Washer incorporates a state-of-the-art, high resolution LED backlit touch screen user interface for intuitive and flexible onboard instrument control of 96- and 384-well microplate washing. A 5.7 inch graphical color display seamlessly guides users through protocol development, instrument maintenance and daily use at the touch of a finger. Two USB flash drive ports enable convenient file transfer, storage and operation. For those who prefer computer control or require 21 CFR Part 11 compliance, the 405 Touch can be controlled with optional Liquid Handling Control™ Software. 

For over 27 years, BioTek has continued to advance the art of automated microplate washing, resulting in the industry leading ELx405™, which set the standard in full 96- and 384-well washing.  The 405 Touch, maintains all the features of the ELx405™ Microplate Washer while adding a new,  modern and powerful interface to again reshape the day-to-day routines of today’s life science laboratories.  Fast and efficient vacuum filtration, high strength biomagnetic separation, patented Dual-Action™ manifolds, optimized cell washing for loosely adherent cell monolayers and patent-pending Ultrasonic Advantage™ are all available.  New key features include automated internal 4-buffer switching and quick release manifolds for easy maintenance and modular use.

Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. BioTek’s washers with their built-in ultrasonic cleaners are the only instruments of their kind available today able to automatically prime with cleaning solution, sonicate manifold tubes to remove protein or salt crystal build up and re-prime with buffer leaving the instrument clean and ready for its next wash. Cleaning these tubes is a time consuming and labor intensive process. BioTek's Ultrasonic Advantage™ incorporates the power of ultrasonic cleaning to make the 405 Touch a self-maintaining microplate washer.

For washing microsphere based assays, all 405 Touch models can be equipped with accessory modules for both vacuum filtration and biomagnetic separation. This allows for full plate washing of polystyrene and magnetic beads used in a growing number of multiplex assays and bead-based ELISAs, such as those developed on the Luminex® xMAP® technology platform. The 405 Touch’s vacuum filtration feature automates the wash steps of filter bottom plates with five different vacuum levels for optimal performance of filter pore sizes ranging from 0.45 µm to 1.2 µm. Developed with leaders in genotyping, gene expression and protein assays, the 405 Touch’s biomagnetic separation incorporates a range of immobilization options each with high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer beads with superior retention. The 405 Touch is Luminex® xMAP® approved.

The 405 Touch Washer, also known as the 405 TS Series, is available in a range of configurations for different microplate formats and wash requirements.  The 405 HT TS is designed specifically to rapidly wash 384-well microplates and includes BioTek's patented Dual-Action™ 192-tube manifold to ensure optimal performance. A proprietary tube-in-tube aspiration manifold design also provides complete 384-well evacuation even with buffers not containing surfactant.  The 405 Select TS offers the patented Dual-Action™ manifold in a 96-tube format, providing independent control of both dispense and aspirate manifolds for precise overfill washing and overflow protection. This unique design washes both 96- and 384-well plates without the need for any hardware changes. With its angled dispense tubes, full control of dispense and aspiration rates and unique X, Y, Z positioning, the 405 Select TS is ideal for the most demanding wash requirements including loosely-adherent cell lines. For those applications that use only 96-well microplates, the standard 405 TS is an ideal choice with unique features to lower background absorbance values and increase the signal-to-noise ratio of typical ELISA assays.

With its color graphical user interface and wide application range, the new 405 Touch Microplate Washer is an easy to use, total solution for 96- and 384-well plate washing in a compact and robot-compatible design. 

Applications:

  • ELISAs
  • MSD assays
  • HCS immune cytochemistry
  • FLIPR® Ca2+ flux
  • Cell-based assays
  • Magnetic and polystyrene bead processing (gene expression assays, cytokine assays)
  • ELISPOT assays

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

 

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

Hardware:

  • Accommodates 96- and 384-well microplates
  • High resolution, LED backlit touch screen UI for intuitive and flexible onboard washer control
  • 5.7 inch graphical color display seamlessly guides users through protocol creation, instrument maintenance and daily use at the touch of a finger
  • Fast Intel Atom processor running Windows CE
  • Resistive touch screen display

    - Supports all gloved environments
    - Compatible with widest range in humidity levels
    - May be operated with pointing devices
    - Low power, green technology

  • Two USB flash drive ports enable convenient file transfer, storage and operation

    - Especially helpful when instrument is shared between researchers
    - Insert memory stick, wash plates…and go
    - Each unit ships with memory stick containing sample protocols and instructional videos

  • 405 HT TS

    - Offers fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection

  • - Includes patent-pending Ultrasonic Advantage™ – built in ultrasonic cleaner for manifold self-maintenance
    - Equipped with superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
    - Also calibrated for accessory 96-tube Dual-Action manifold for 96-well washing

  • 405 Select TS

    - Accommodates 96-/384-well washing with patented 96-tube Dual-Action™ manifold
    - Includes optimized cell washing with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers

  • 405 TS models automate basic 96-well dispensing and aspiration
  • Automated internal 4-buffer switching
  • Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
  • Inline filtration within each dispense bottle
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
  • All washers vacuum filtration ready (accessory filtration modules sold separately)
  • All washers biomagnetic separation ready (accessory magnets sold separately)
  • Quick change manifold designs
  • Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
  • BioStack™ Microplate Stacker compatibility for batch processing of up to 50 ANSI/SBS standard height plates

Software:

  • Context sensitive help system complete with recommendations, diagrams and images is only a key press away when questions arise
  • Instructional videos walk users through instrument setup and basic use
  • Quick menu allows fluid paths to be easily primed, wash process started and manifold cleaned
  • Predefined protocols included for out-of-box washing

    - ELISA
    - Cell washing
    - Vacuum filtration and biomagnetic separation – Luminex® xMAP® technology workflows

  • Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays

    - Dispense height
    - Flow rate
    - Aspiration height
    - Soak time
    - Shake time and frequency
    - Aspiration rate
    - Horizontal dispense and aspirate positions
    - Crosswise aspiration

  • AutoPrime
  • Additional software flexibility and 21 CFR Part 11 compliance with Liquid Handling Control™(LHC) Software for method development, downloading and washing plates.
  • Run protocols created onboard, downloaded from LHC™ Software or transferred on USB drive
Modello
Codice
96/384 pozzetti
384 pozzetti
96 pozzetti
Lavaggio cellulare
Switch soluzioni tampone
Ultrasuoni
Separazione biomagnetica*
Filtrazione a vuoto*
405™ HT TS
405TSHT
      
405TSHTV
   
405™ Select TS
405TSU
    
   
405TSUV
405TSUS
   
 
405TSUVS
   
405™ TS
405TSR
 
 
405TSRV
    
 
 
405TSRS
   
 
405TSRVS
   
 

* Con moduli accessori venduti separatamente

 

Configurazioni del lettore per micropiastre 405™ Touch
Caratteristiche Design
HT Lavaggio rapido di micropiastre a 384 pozzetti
  • Manifold 192 tubi (16 x 12)
  • Dual-Action™
V Switch automatico interno per 4 soluzioni tampone  
S Ultrasonic Advantage™
  • Lavaggio ad ultrasuoni incorporato per l'automanutenzione del manifold
U Lavaggio di micropiastre a 96/384 pozzetti
  • Manifold 96 tubi (8x12)
  • Dual-Action™
R Lavaggio di micropiastre a 96 pozzetti
  • Manifold a 96 tubi (8x12)

 

Generale
Test ELISA
Bead magnetiche e in polistirene
  • Test multiplex
  • ELISA con bead


  • Processi dalla filtrazione allo scarico
Separazione Separazione biomagnetica, filtrazione a vuoto
Tipi di micropiastre 96 e 384 pozzetti (in funzione del modello)
Basso profilo ed altezza standard
Fondo solido o filtrante
  • Filter pore sizes 0.45 µm to 1.2 µm
Agitazione Programmabile in minuti e secondi, fino a 60 minuti
Intensità - lenta, media, veloce o variabile
Tempo d'immersione Programmabile in minuti e secondi, fino a 60 minuti
Magnete Ad alta potenza per micropiastre a 96 e 384 pozzetti
Scelta tra due design (personalizzazione dei lavatori di micropiastre BioTek):

- Lineare
- Pozzetti a fondo piatto - bead attratte in bande sul fondo del pozzetto
- Pozzetti a fondo arrorotondato - bead spinte ad agglomerarsi sul fondo del pozzetto

- Ad anello - bead attratte in 4-zone ad anello sul fondo del pozzetto
Sicurezza/Convenienza/Manutenzione Sensore di livello dei liquidi, dello scarico, del vuoto, del flusso
Coperchio per aerosol
Utility per la regolazione del posizionamento della micropiastra
Protezione per il sovraccarico
Procedure di manutenzione preprogrammate
Automazione Impilatore di micropiastre BioStack™ - fino a 50 micropiastre ANSI/SBS di altezza standard(opzionale)
  • Escluse micropiastre con fondo filtrante
Software integrato Touch screen a LED, ad alta risoluzione retroilluminato
Schermo grafico a colori da 5,7 pollici
Processore Intel Atom con Windows CE
Protocolli di lavaggio predefiniti - ELISA, lavaggi cellulari, lavaggi con bead per separazioni biomagnetiche e filtrazione a vuoto
Menu rapido
Creazione o modifica di più protocolli personalizzati
Esecuzione di protocolli creati direttamente sullo strumento, scaricati dal software LHC ™ o trasferiti via chiavetta USB
Software Software Liquid Handling Control™ (LHC™), per gestire ed eseguire i protocolli di lavaggio da PC (opzionale)
Temperatura operativa Da 15° C a 30° C (da 59° F a 86° F)
Interfaccia Per controllo da PC ed integrazione con BioStack™ – 1 porta USB, 1 porta RS232 a 9 pin
Per il trasferimento di file – 2 porte di memoria USB
Umidità Da 10% a 85%, non-condensante
Elettrico 100 – 240 Volt AC 50/60 Hz, < 8.0 A
Lavaggio
Tipi di manifold Lavaggio di micropiastre da 96 pozzetti:
Manifold da 96 tubi (8 X 12)
Lavaggio di micropiastre da 96/384 pozzetti:
Manifold Dual-Action™ da 96 tubi (8 X 12)
Lavaggio di micropiastre da 384 pozzetti:
Manifold Dual-Action™ da 192 tubi (16 x 12)
Velocità di lavaggio Micropiastre con fondo solido:

3 cicli di asp./disp.: 300 µl/pozzetto, 96 pozzetti, manifold a 96 tubi, aspirazione finale: ≤ 30 secondi
3 cicli di asp./disp.: 100 µl/pozzetto, 384 pozzetti, manifold a 96 tubi, aspirazione finale: ≤ 80 secondi
1 ciclo di asp./disp.: 400 µl/pozzetto, 384 pozzetti, manifold a 192 tubi, aspirazione finale: ≤ 20 secondi

Micropiastre con fondo filtrante:
Variabile, in funzione dei parametri di lavaggio
Distribuzione del liquido Pompa interna a spostamento positivo
Filtrazione sotto vuoto Livelli di vuoto selezionabili (finali circa a 30 secondi):

Minimo: -38 mmHg
Basso: -113 mmHg
Medio: -200 mmHg
Alto: -390 mmHg
Massimo: -506 mmHg

Intervallo tempi della filtrazione a vuoto:
da 5 a 999 secondi
Gamma dei volumi Da 25 a 3000 µL/pozzetto
Selezionabile in incrementi di 1 µL
Selezione del tampone Switch interno automatico fino a 4 soluzioni tamponi (opzionale)
Velocità del flusso 1-11
Incluso il flusso basso, ed i l lavaggio di cellulle (a seconda del modello)
Cicli di lavaggio Da 1 a 250
Precisione di dispensazione
  • Lavaggio micropiastre da 96 pozzetti - manifold a 96 tubi (8 x 12):
    3% CV: 300 µL/pozzetto (acqua deionizzata con 0,1% di Tween 20 e FD & C # 1 colorante blu), tasso 6, assorbanza letta a 630 nm e 450 nm di riferimento
  • Lavaggio micropiastre da 384 pozzetti - manifold a 192 tubi (16 x 12):
    <4% CV: 80 µL/pozzetto (acqua deionizzata con 0,1% di Tween 20 e FD & C # 1 colorante blu), tasso 7, assorbanza letta a 630 nm e 450 nm di riferimento
Volume residuo Micropiastre con fondo solido:
  • Lavaggio di micropiastre da 96 pozzetti - manifold a 96 tubi (8 x 12):
2 µL/pozzetto: 3-cicli di lavaggio, 300 µL/pozzetto (acqua deionizzata con lo 0,1% di Tween 20), micropiastra Corning da 96 pozzetti a findo piatto, altezza d'aspirazione ottimizzata

  • Lavaggio di micropiastre da 384 pozzetti - manifold a 192 tubi (16 x 12):
< 2 µL/pozzetto: 3-cicli di lavaggio, 100 µL/pozzetto(acqua deionizzata con lo 0,1% di Tween 20),micropiastra Corning da 96 pozzetti a findo piatto, altezza d'aspirazione ottimizzata

Filter bottom plates:
  • 96-well:
Average increase weight of plate < 1.2 g after dispensing 300 µL to
0.45 µm plate, filtration 30 seconds, low vacuum, blotted
  • 384-well:
Average increase weight of plate < 4.0 g after dispensing 80 µL to 1.2
µm plate, filtration 10 seconds, low vacuum, blotted
Sterilizzazione Compatibilità chimica
0,5% ipoclorito di sodio, 70% alcol isopropilico o 70% di soluzione di etanolo
Pulizia ad ultrasuoni Bagno ad ultrasuoni integrato per rimuovere accumuli di proteine e cristalli di sale.
Programmabile in ore e minuti, fino a 60 minuti (in base al modello)
Volume bottiglia di rifornimento 4 L o 10 L (opzionale)
Caratteristiche fisiche
Dimensioni Profondità: 17" (43.2 cm)
Larghezza: 14" (35.6 cm)
Altezza: 10" (25.4 cm)
Peso Con switch interno - 36 lbs (16,5 kg)
Senza - 30 lbs (13,5 kg)
Regolamentazione
Regolamentazione Per uso diagnostico in vitro. Tutta la strumentazione Biotek per micropiastre è marcata CE e TUV.

Le specifiche sono soggette a modifica.

Opzionale

BioStack - sistema robotico per lo stoccaggio di micropiastre

BioStack - sistema robotico per lo stoccaggio di micropiastre

BioStack™ Microplate Stacker adds stacking capabilities to BioTek's Synergy™, PowerWave™, 405™, ELx405™ Select Deep Well, EL406™, MultiFlo™ and Precision™ product families.


Software Liquid Handling Control

Software Liquid Handling Control

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


Standard (incluso)

Manuale utente

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Opzionale

Kit di integrazione per il Biostack

7310035 BioStack Integration Kit for 405™ TS and 405™ LS

Bottiglie e tubistica

1173000 Dispense Bottle - with Filter, 10L
1173002 Cap for Dispense Bottle - with Filter, 10L
1173003 Cap for Dispense Bottle - with Filter, 4L
1173031 Dispense Bottle – with Filter, 4L
7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) l'accessorio ha un'immagine
7100533 Waste Tubing Set l'accessorio ha un'immagine
7100534 Waste Bottle, 4L l'accessorio ha un'immagine
7100537 Dispense Tubing Set – 4 Buffers
7100538 Dispense Tubing Set –1 Buffer l'accessorio ha un'immagine
7100542 Waste Bottle with Level Sensing, 4L l'accessorio ha un'immagine
7100543 Waste Set with Level Sensing, 4L Bottles l'accessorio ha un'immagine
7100544 Cap for Waste Bottle with Level Sensing, 4L l'accessorio ha un'immagine
7100556 Waste Bottle and Tubing, 20L l'accessorio ha un'immagine
7100557 Waste Bottle and Tubing, 10L l'accessorio ha un'immagine

Sistema di dispensazione/scarico

1170528 Complete Dispense System, 10L Bottles
1170529 Complete Dispense System, 4L Bottles
1170530 Complete Dispense/Waste System 115V/230V, 4L Bottles
1170531 Complete High Flow Waste System 115V/230V, 10L Bottles
1170532 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles
1170533 Complete Dispense/High Flow Waste System 115V/230V, 10L Bottles
1170534 Complete Waste System 115V/230V, 10L Bottles
1170535 Complete Dispense/Waste System 115V/230V, 10L Bottles
1170536 Complete Dispense/Direct Drain Waste System 115V/230V, 4L Bottles
1170537 Complete Dispense/Direct Drain Waste System 115V/230V, 10L Bottles
7100582 Vacuum Set with Level Sense Assembly, 10L l'accessorio ha un'immagine
7100746 Complete Waste System 115V/230V, 4L Bottles l'accessorio ha un'immagine
7100753 Complete High Flow Waste System 115V/230V, 4L Bottles
7103024 Standard Vacuum Pump 115V/230V l'accessorio ha un'immagine

Filtri

01310 Filter for Dispense Bottle
48294 Filter for Waste Tubing l'accessorio ha un'immagine

Manifold

1170010 96-tube Dual-Action™ Manifold Kit – with 7° Dispense Manifold and Tube - in - Tube Aspiration Manifold
1172046S 96-tube Dual-Action™ Tube-in-Tube Aspiration Manifold
7102068S 96-tube Dual-Action™ 7° Dispense Manifold

Varie

01113 Silencing Muffler for Standard Vacuum Pump
1170008 96-well Vacuum Filtration Kit
1170009 96-/384-well Vacuum Filtration Kit
4030551 Vacuum Gauge/Regulator
7100025 Stylus Kit for any 405™/ELx405™ Select Deep Well manifold l'accessorio ha un'immagine
7102215 384-well Ring Magnet l'accessorio ha un'immagine
7102216 96-well Ring Magnet l'accessorio ha un'immagine
7103016 96-well Flat Magnet l'accessorio ha un'immagine
7103017 384-well Flat Magnet l'accessorio ha un'immagine

Pacchetto IQ/OQ

1170522 405™ TS Qualification Package - IQ/OQ/PQ package on CD

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