Cell Metabolism

Cell StructureCellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.  BioTek’s imaging products Cytation™ 1 and Cytation 5 can be used to normalize Agilent’s Seahorse OCR and ECAR replicate data; while our microplate readers, Synergy™ H1, Synergy Neo2 and Cytation all containing time-resolved fluorescence modules can be used to read Agilent’s soluble sensor family of products.

 

Cell Metabolism: Seahorse XF Imaging and Normalization

Agilent Technologies offers an integrated imaging and normalization system which combines cellular analysis and imaging for an intuitive workflow to streamline your XF data interpretation. This imaging and normalization solution combines Agilent's strength in cellular metabolic analysis with BioTek's capability in cell imaging.   

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Cell Metabolism: MitoXpress and pH-Xtra Assays

Agilent’s MitoXpress and pH-Xtra reagent kits enable simple mix and measure assays that utilize soluble, fluorescent nano sensor probes to measure oxygen consumption rates (OCR) and extracellular acidification rates (ECAR). These sensors were designed to provide maximum ease-of-use, higher throughput, and multiplexed data markers for kinetic metabolism assays.  

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Learn more about Cell Metabolism applications - search our Application Notes and technical documentation in Resources.

 

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