Cell Stress
Endoplasmic reticulum (ER) mediated cell stress and the unfolded protein response (UPR) serve as a major stress pathway in eukaryotic cells. ER stress and the UPR can be triggered by various cellular perturbations including misregulation of ER Ca2+, accumulation of misfolded proteins within the lumen of the ER, and reactive oxygen species. Activation of proteostatic pathways, such as the UPR and ER stress serves as an early indicator of treatment-induced cell stress or toxicity. Combining automated live cell imaging and analysis with the reversible cell stress biosensor from Montana Molecular provides a sensitive and reversible method. This method is ideal for detecting the UPR and ER stress in real time.
Figure 1. Detection of UPR and ER stress over time.
Related Resources:
- Thapsigargin-induced Cellular Stress Response and Inhibition of Gq-dependent Calcium Signaling
- Monitoring Cellular Stress Response and Inhibition of GPCR-Dependent Calcium Signaling Using Expressed Biosensors
- Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43
Related Products:
- Agilent BioTek Lionheart FX Automated Microscope
- Agilent BioTek BioSpa Live Cell Analysis System
- Agilent BioTek Cytation 7 Cell Imaging Multi-Mode Reader
- Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader
- Agilent BioTek Cytation 1 Cell Imaging Multi-Mode Reader
Learn more about Cell Stress applications - search our application notes and technical documentation in Resources.