Directed Activation of T Cells for Cytotoxicity Assays
T cells are critical to the adaptive immune system as they recognize and destroy pathogenic cells while leaving healthy cells unharmed. As such, they are a promising focus for novel cancer therapies. Key processes in these T cell immunotherapies are the directed activation and expansion of a patient’s own T cells in vitro. The activation proceeds through the addition of antigen(s), and often, IL- 2. However, in order to strengthen the response by directing the activation, and better mimicking in vivo behaviors, the T cells may be cultured in the presence of target cells, which directs activation towards target cell antigens.
The activation process occurs over multiple days in microplates and is amenable to automation, where liquid handlers like MultiFlo™ FX Multi-Mode Dispenser can be used to seed both target and T cells and its AMX module deployed to automatically perform media exchanges over the course of the activation without loss of the non-adherent T cells. The Cytation™ Cell Imaging Multi-Mode Reader, inconjunction with BioSpa™ 8 Automated Incubator can be used to periodically image both target and T cells during the activation process.
Directed T cell activation in a microplate well imaged in brightfield using Cytation.