Total Protein Quantification
Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional absorbance-based colorimetric methods to measure total protein content.
Total protein is quantified using the same methods as nucleic acids:
- Spectrophotometric determination of protein and peptides at A280
- Colorimetric determination with Bradford, Lowry and BCA assays, for example
- Fluorometric determination using intrinsic fluorescence or fluorescent probes eg. NanoOrange, CBQCA, Fluorescamine
Total protein quantification can routine be run in a variety of labware on Agilent BioTek instruments, including: microplates, Agilent BioTek Take3 microvolume plates, cuvettes and BioCell.
Application Notes:
- Fluorometric Protein Quantification using NanoOrange
- Green Fluorescent Protein Quantification in Microplates
Learn more about Total Protein Quantification – see our applications and technical documentation in resources.
Related Products:
- Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader
- Agilent BioTek Cytation 1 Cell Imaging Multi-Mode Reader
- Agilent BioTek Synergy Neo2 Hybrid Multi-Mode Reader
- Agilent BioTek Synergy H1 Hybrid Multi-Mode Reader
- Agilent BioTek Synergy HTX Multi-Mode Reader
- Agilent BioTek Synergy LX Multi-Mode Reader
- Agilent BioTek Epoch 2 Microplate Spectrophotometer
- Agilent BioTek ELx808 Absorbance Reader
For Research Use Only. Not for use in diagnostic procedures.
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