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Investigation of Cell Migration in a Label-free Cell Exclusion Assay by Automated Microplate Imaging

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February 06, 2019

Authors: Joe Clayton, Pete Brescia and Peter Banks, BioTek Instruments, Inc., Winooski, VT, USA

Introduction

  • Cell migration is the movement of cells from one location to another, driven by complex environmental signaling events and induced structural changes to the cytoskeleton.
  • Multicellular organisms rely on cell migration for a vast array of biological processes, including embryonic development, immune responses, wound healing, and cancer metastasis.
  • The need to better understand the mechanisms that regulate cell migration has led to the development of improved methods of investigation, including specialized cell culturing platforms and live-cell imaging techniques.
  • Here we demonstrate an automated kinetic imaging-based approach to investigating cell migration using the Oris™ Cell Migration Assay Kits.
  • This convenient label-free method enables accurate and robust analysis of cell migration within a 96- or 384-well platform for high-throughput applications.
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