File di esempio
Solution ELISA Prozone and Sensitivity assay
June 05, 2013Download
Related Products: Gen5 for Detection, Lettore ibrido multi-mode Synergy Neo2
Related App Note: Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products
Gen5 version required: 2.0 or higher
Gen5 was used to determine luminescence signal in a solution ELISA assay for evaluation of the sensitivity and prozone effect of the positive control used during immunogenicity testing for anti-drug antibodies. The positive control was serial diluted to evaluate the lowest concentration meeting the acceptance criteria for intra-and inter-assay precision and the ability to detect ~5% false positives among samples tested. The sample file contains the read parameters used for this experiment. Data was collected and exported to Microsoft Excel for further statistical analysis.