Prodotti
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- Imaging & microscopia
  Automated Cell Imagers
  
  Lettore di imaging per cellule Lionheart FX
  Lionheart LX Automated Microscope
  Letture multi-mode per imaging cellulare
  
  Cytation 7, lettore multimode imaging cellulare
  Cytation 5, lettore multimode imaging cellulare
  Cytation 1, lettore multimode imaging cellulare
  Multi-Plate Live Cell Imaging System
  
  BioSpa Live Cell Analysis System
  Peripherals
  
  Cell Count & Viability Starter Kit
  Scratch Assay Starter Kit
  Gas Controller
  Dual Reagent Injector Module
  Scheda panoramica dei prodotti
  
  Imaging & microscopia
  
  Software Gen5
- Lettura
  Lettori di micropiastre multimode con tecnologia ibrida
  
  Lettore di micropiastre multi-mode Synergy Neo2
  Cytation, lettore multimode
  Lettore di micropiastre multi-mode Synergy H1
  Lettore di micropiastre multi-mode Synergy HTX
  Lettore multi-mode Synergy LX
  Lettori in assorbanza
  
  Spettrofotometro per micropiastre Epoch 2
  Spettrofotometro per micropiastre Epoch
  Lettore di micropiastre in assorbanza ELx808
  Lettore di micropiastre in assorbanza 800 TS
  Peripherals
  
  Gas Controller
  Take3 Plate
  Dual Reagent Injector Module
  Multi-Plate Absorbance Reader
  
  LogPhase 600 Microbiology Reader
  Scheda panoramica dei prodotti
  
  Lettori multi-mode
  
  Microplate Readers
  
  Microplate Spectrophotometers
  
  Software Gen5
- Lavaggio/dispensazione
  Multi-Mode Washer Dispensers
  
  EL406 combinazione lavatore-dispensatore
  Dispensatore multi-mode MultiFlo FX
  Lavatori di micropiastre
  
  Lavatore di micropiastre 405 TS
  Lavatore di micropiastre 405 LS
  Lavatore di micropiastre ELx405
  Lavatore a strip di micropiastre 50 TS
  Dispensatori di micropiastre
  
  Dispensatore multi-mode MultiFlo FX
  Dispensatore per micropiastre MicroFill
  Product Comparison Charts
  
  Microplate Washers
  
  Microplate Dispensers
- Robotica
  Robotica
  
  Incubatore automatizzato BioSpa 8
  BioStack - sistema per lo stoccaggio di micropiastre
  BenchCel Microplate Handler
  Product Comparison Charts
  
  BioStack Configuration Comparison
- Buy Cell Culture Microplates
  Sourcing your microplates shouldn't be an added task in preparing your cell-based workflows. Agilent now offers cell culture and imaging microplates so you can complete your workflows seamlessly.
  
  Click here to request your free sample.
- Software
  Software
  
  Gen5 Software Features for Imaging & Microscopy
  Software di analisi dati Gen5
  Software Liquid Handling Control
  Software Apps
  
  LogPhase 600 App
  Cell Count & Viability App
  Scratch Assay App
  Quantitative Assay Apps
  Product Comparison Charts
  
  Gen5 Software Version Comparison
- Technologie
  Multi-Mode Detection Technologies
  
  Absorbance
  Alpha Detection
  Filtered Luminescence
  Flash Luminescence
  Fluorescence Intensity
  Fluorescence Polarization
  Glow Luminescence
  Time Resolved Fluorescence
  TR-FRET
  Imager & Microplate Reader Technologies
  
  4-Zone Incubation and Condensation Control
  Augmented Microscopy
  Hybrid Technology
  Laser Autofocus
  Peltier Cooling Module
  Variable Bandwidth Monochromators
  Liquid Handling Technologies
  
  AMX Technology
  Dual-Action Manifold
  Parallel Dispense
  RAD Technology
  Ultrasonic Advantage
  Verify Technology
- Richiedi un preventivo
  
  To request pricing or a quote, use our configurator to assist you in choosing the appropriate instrument part # and accessories to meet your needs.
Peripherals
- Peripherals
  BioTek offers many peripheral instruments and accessories to expand functionality and enhance assay workflows. Many peripheral instruments and modules are compatible with several different instruments, see the list here.
- Accessori
  To complete or enhance your BioTek imager, microplate reader, liquid handling or robotics system, we offer a wide range of accessories, from tubing and bottles to imaging filter cubes. Search our Accessory database for Accessories compatible with your instrument. Search by Keyword, Part #, Product or Accessory Type.
- Application Starter Kits
  BioTek’s Application Starter kits contain everything you’ll need to implement the latest assays for a particular application, quickly and with minimal training. Kits contain hardware, software, consumables and reagents all in a convenient kit.
Applicazioni
- Cell Culture QC
  
  Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points.
- Cell Health and Viability
  
  Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury.
- Cell Metabolism
  
  Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.
- Migrazione/invasione cellulare
  
  Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models.
- Developmental Biology
  A macro trend in drug discovery is the use of complex models of disease early in preclinical studies. An approach following this viewpoint is the use of whole organism models involving living invertebrates and vertebrates such as nematodes and zebrafish conducive to drug screening methods.
- Histology and Slide Scanning
  Histology is typically performed on fixed tissue samples using upright microscopes equipped with oculars. Cytation™ and Lionheart™ FX provide automated digital imaging of these samples using color brightfield microscopy. Low to high magnifications from 1.25x to 60x (100x with Lionheart FX) can be combined with Cytation Gen5™ image processing features such as image stitching to increase field of view.
- Immunology
  The immune system relies on diverse mechanisms working in concert to defend the host from infection and to identify and remove aberrant or damaged cells. Dysregulation of the immune system can lead to a variety of disease conditions, including autoimmune disorders, allergies, and cancer. Additionally, the immune system is a critical consideration for vaccine development and cell, tissue and organ transplants.
- Immuno-Oncology
  While harnessing the patient’s immune system to fight cancer is not a new concept, there have been significant strides in the past decade to more effectively direct and activate components of the immune system and counter the measures certain tumors adopt to shield itself from immune attack. These include immunotherapeutic drugs based on directing immune cells to tumors based on specific antigens they present, PD-1 checkpoint inhibition and the ex vivo manipulation of the patient’s own immune cells to better drive an immune response to cancer.
- Microbiology
  Since the advent of a lens system that enabled the visualization of bacteria and protists in 1671, the microscope remains a ubiquitous tool for the scientific study of live and fixed microorganisms. Investigating the structure, function, and typing of the highly diverse array of microorganisms spans heterogeneous applications including, but not limited to, the elucidation, diagnosis, and treatment of human and plant disease, food and water safety testing, vaccine development, investigation and production of industrial lipases, protein transfection to mammalian cells, environmental health and impact appraisal, horticultural and agricultural soil assessment, and exploration of alternative fuel sources.
- Molecular Biology and Biochemical
  Biochemical assays run the gamut from routine ELISAs to micro-volume nucleic acid and protein quantification. Endpoint and kinetic, single- and multi-wavelength assays are essential experimental tools. BioTek’s multi-mode readers have the flexibility to accommodate a very broad range of applications with superior performance.
- Rapid Cell Signaling
  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
- Stem Cell Research
  Research into the reprogramming of somatic cells into pluripotent stem cells received the Nobel Prize in 2012. The long term importance of this research resides in the use of stem cells for regenerative therapies; for the short term, however these cells represent a potential unlimited source of highly physiologically relevant cells, akin to human primary cells, that can be used as drug discovery reagents.
- Virology
  Research into the diverse aspects of virology are made possible by a broad range of applications and assay formats, from relatively simple methods for viral detection to those designed to elucidate complex immune responses and pathological processes. BioTek instruments provide versatile platforms for conducting viral research, with optimized imaging- and plate reader-based solutions, as well as liquid handling and automation tools for increased assay throughput and reproducibility.
- Detection & Liquid Handling
  Detection & Liquid Handling
  
  Saggi con biomarcatori
  Cell-based Assays
  Crescita cellulare e microbica
  Saggi ELISA
  Quantificazione degli acidi nucleici
  Quantificazione delle proteine
Risorse tecniche
- Note applicative
  
  BioTek’s application notes present the latest-breaking scientific content covering the vast range of applications across our entire product line. Browse applications by topic, detection and product or use the simple keyword search to find the application notes that will facilitate your research.
- Application Bulletins
  
  BioTek’s application bulletins provide brief, concise descriptions of timely applications enabled with BioTek instrumentation.
- Guida applicativa
  
  Many applications are straightforward and need no explanation, but best practices and tips & tricks are useful for many complex or esoteric workflows. BioTek’s application guides offer detailed information on a variety of topics.
- Pubblicazioni
  Search or browse our database to find scientific articles submitted by BioTek’s applications scientists and published in leading journals.
- Poster Presentazioni
  BioTek’s application team has developed more than 170 scientific posters on their latest research highlighting the applications enabled with our life science instrumentation. Browse or search the topics or products of interest.
- Note tecniche
  
  Our Technical Notes illustrate product-specific capabilities and common applications for BioTek microplate instrumentation, and their implementation in common experimental design.
- Webinars
  Our webinar archives are available for on-demand viewing - search to find a topic, application or product of interest. You can also register to be invited to future webinars here.
- Visual Abstracts
  BioTek’s Visual Abstracts are brief animated presentations describing the workflow of a single specific imaging or multi-mode detection application.
- Dossier aziendali
  Browse BioTek’s white papers for timely information on such important topics as Reactive Oxygen Species and 3D Cell culture.
- Citazioni
  
  Search the more than 3,000 scientific citations for BioTek’s products within our database to learn more about the applications for our life science instrumentation as they’re used around the world.
- Video
  Here you will find a collection of videos found throughout our site. These videos can also be found on BioTek's YouTube channel.
- Upcoming Webinars
  Through our Webinar presentations, BioTek strives to share information and experiences about timely and important microplate-based applications. Sign up today and check back for future topics that may be of interest to you.
Supporto e assistenza
- Supporto tecnico
  The BioTek Global Technical Support Center (GTS) is your Help Desk for all questions related to BioTek product use, maintenance, troubleshooting support and other frequently asked questions. Normal GTS hours are weekdays from 8:30 A.M. - 5:30 P.M Eastern Standard Time, excluding holidays.
- Field Applications Scientists
  BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software.
- Rete di assistenza clienti
  BioTek Instrument Service and Repair is available worldwide at several of our International Service Centers. Field Service is also available for many customers throughout the world.
- FAQ
  Search our Frequently Asked Questions by keyword, product or category. For additional assistance call (800) 242-4685 or submit a question via our Ask a Question form.
- Centro risorse clienti
  BioTek's Customer Resource Center (CRC) gives customers access to information about their specific BioTek instrumentation and software. Register and log in to:
  
  - Check status and track orders
  - Manage equipment inventory
  - Access documentation, user manuals and software
  - Request Service & Support
- Richiesta Assistenza
Chi siamo
- News
  Synergy H1’s Continuously Variable Bandwidth Monochromators Optimize Assay Performance
  July 2, 2020 - BioTek Instruments announces the availability of variable bandwidth monochromators on their modular and versatile Synergy™ H1 Hybrid Multi-Mode Reader. This allows researchers to achieve even greater levels of assay sensitivity and specificity compared to fixed bandwidth systems. Read more
  
  LogPhase 600 Microbiology Reader Measures Microbial Growth Curves in Multiple Microplates
  June 9, 2020 - BioTek unveils a unique class of microplate readers with the new LogPhase 600 Microbiology Reader. The LogPhase 600 is the only four-plate microplate reader purpose-built to perform microbial growth curve analysis. Multiple plate measurements increase throughput and reduce the need for multiple costly single-plate absorbance readers that take up valuable bench space. At the same time, highly consistent environmental conditions support high quality absorbance results without growth curve artifacts, even in bacterial and yeast assays with extended incubation periods. Read more
  
  Agilent’s BenchCel Microplate Handler Now Available through BioTek for Full Workflow Automation
  May 28, 2020 - BioTek announces that Agilent’s BenchCel Microplate Handler is now added to its portfolio of walkaway automated solutions. The modular BenchCel Microplate Handler is a high-speed robot with an open, flexible and scalable format to bring efficiency to diverse applications such as ELISA, endpoint add-and-read assays and cell fix-stain-image processes. Read more
  Più news
- Eventi
  SLAS2021 Digital International Conference and Exhibition
  
  January 23 - 27, 2021
  
  BioTek instrumentation will be on display by BioTek U.S.
  
  Online
  
  AACR 2021
  
  April 10 - May 21, 2021
  
  Virtual Meeting
  Week 1: April 10-15; Week 2: May 17-21
  
  BioTek instrumentation will be on display by Agilent Technologies.
  
  Online
  
  Più eventi
- Pubblicità clienti
  Nothing can speak to the experience you'll encounter with BioTek like stories from our customers themselves. Customer satisfaction is our top priority. From Engineering to Manufacturing to Sales and Support, BioTek employees around the world are dedicated to providing the best product and best customer experience in the industry.
- Riconoscimenti
  See some of the many awards that BioTek and our products have received over the years.
- Storia
  At BioTek we feel passionately and genuinely that our employees are our greatest asset. We continue to build our already impressive team having doubled our global workforce in the last five years. With our headquarters in the beautiful Green Mountains of Vermont, USA, we also have regional offices around the world.
  
  If you're interested in becoming part of our amazing team, check out our list of job openings today!
- Partner
  As a prospective partner, BioTek can help you achieve your sales and market penetration by providing a unique blend of products, service, support, training, co-marketing and quality assurance designed to meet your customers’ requirements while you remain focused on your core business.
- TekTalk Newsletter
  Review past issues of TekTalk, BioTek's complimentary quarterly e-newsletter. If you are not currently subscribed, you may sign up now to begin receiving the latest life science instrumentation news.
- Blog
  Visit our blog, Scientifically Speaking, to read what our scientists and instrument experts have to share.
- Carriere
  At BioTek we feel passionately and genuinely that our employees are our greatest asset. We continue to build our already impressive team having doubled our global workforce in the last five years. With our headquarters in the beautiful Green Mountains of Vermont, USA, we also have regional offices around the world.
  
  If you're interested in becoming part of our amazing team, check out our list of job openings today!
- Quality and Compliance
  BioTek is committed to maintaining the high quality of our products and services. All of our products are designed and manufactured in the USA at our Headquarters in Winooski, Vermont. Our continual improvement process includes soliciting customer input on quality and product features, and then designing, validating, manufacturing, and testing products to ensure performance and reliability.
- Contattaci
  Contact BioTek today. Ask a sales or technical question or request service.

Risorse - Webinar

ELISA: Best Practices and Troubleshooting - On Demand

Related Products: Lavatore 405 LS, Lavatore 405 TS , 50 rondelle TS , 800 TS Reader di assorbanza, Incubatore automatico BioSpa 8 , Sistema per l'imaging di cellule in vivo BioSpa , Sistema per lo stoccaggio di micropiastre BioStack, Lettore ibrido multi-mode Cytation, Lavatore dispensatore EL406 , ELx405, Lettore di assorbanza ELx808, Gen5 for Detection, Software Liquid Handling Control, Synergy H1, Lettore multi-mode Synergy HTX , Lettore ibrido multi-mode Synergy Neo2

September 20, 2017

Presenter: Dr. Paul Held - Applications Laboratory Manager, BioTek Instruments

Enzyme-linked immunosorbent assay (ELISA) is an antibody-based reaction that allows the detection and quantitation of numerous analytes with the same basic assay process. The reaction uses a solid surface, typically a microplate, to immobilize the analyte. Once immobilized, unwanted materials can be removed by washing and the remaining analyte quantitated. The assay technology uses antibodies in conjunction with enzyme-catalyzed amplification to provide high specificity and high sensitivity. Here we will describe the basic process steps of ELISA, best laboratory practices along with troubleshooting tips for common problems. In addition, some of the devices and instrumentation used with this assay technology will be described.

link

January 23 - 27, 2021

April 10 - May 21, 2021

Risorse - Webinar

ELISA: Best Practices and Troubleshooting - On Demand